Carey M., Smale S. Transcriptional regulation in eukariotes. Concepts, strategies and techniques

2000 Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Despite the global interest in elucidating mechanisms of transcriptional regulation, a comprehensive source of strategic, conceptual, and technical information has not been available for those entering the field for the first time. Although protocols for numerous techniques have been published, the strategic decisions necessary to carry out a step-by-step analysis have not been outlined. This deficiency became apparent to us while we were serving as instructors for the Eukaryotic Gene Expression course held each summer at Cold Spring Harbor Laboratory. This laboratory course was designed for physician-scientists interested in understanding the regulation of a specific disease-related gene, Ph.D. scientists trained in other fields who became interested in the regulatory mechanisms for a gene involved in a particular biological process, and graduate students or postdoctoral fellows who were initiating transcriptional regulation projects. This book is targeted toward this same diverse group of scientists who have developed an interest in transcriptional regulation.