Diaconu M. Laccase-MWCNT-chitosan biosensor-A new tool for total polyphenolic content evaluation from in vitro cultivated plants

Sensors and Actuators B: Chemical, 2010, V. 145, 800-806 pp
In present work, laccase from Trametes versicolor, an enzyme with broad substrate specificity for the phenolic substrates was employed as a biorecognition element in order to develop a biosensor for total content evaluation of polyphenolic secondary metabolites from two in vitro cultivated plants: Salvia officinalis and Mentha piperita. The enzyme immobilization was achieved by entrapment into the nanocomposite film during the electrodeposition process from multiwall carbon nanotubes (MWCNT)–chitosan (CS) solution.