Afify Said M., Seno Masaharu. Methods in Cancer Stem Cell Biology

Springer, 2023. — 251 p. — ISBN 978-981-99-1330-5.This book describes the use of stem cells and cancer stem cell generation in the inflammatory microenvironment (cancer-inducing niche) using induced pluripotent stem cells. It provides step-by-step techniques and manuals for studying stem cell and cancer stem cell generation with different applications in cancer research. The development of induced pluripotent stem cells has provided a new approach to studying cancer initiation by producing cancer stem cells without introducing mutations or foreign genes. The book features the research of the authors’ group, which was the first to generate cancer stem cells from stem cells in the presence of inflammatory conditions. The 20 chapters of this book cover topics such as generating pluripotent stem cells, converting normal stem cells to cancer stem cells, enriching, isolating and evaluating cancer stem cells. Methods for evaluating the characteristics of cancer stem cells and possible therapies against them are also discussed. The book provides easy-to-follow protocols that help researchers in the study of cancer stem cells. Illustrations help readers understand how the method of cancer stem cell generation can be applied as an essential method for assessing the carcinogenic potential of various non-mutagenic compounds. It will be a useful resource for graduate students, researchers, technicians, and physicians working in academic, hospital, and pharmaceutical settings.On the Origin of Cancer
Culture of Cells: Basic Principles
Stem Cell Culture from Embryos
Reprogramming of Normal Cells into Human Pluripotent Stem Cells
Maintenance of Human Pluripotent Stem Cells
Identification of Cancer Stem Cells by Different Molecular Markers
Enrichment of Cancer Stem Cell from Malignant Tumor
Isolation of Single Clonal Cell from Primary Cultured Cells and Establishment of a Cancer Stem Cell Line
Artificial Generation of Cancer Stem Cells from Human Stem Cells
Quick Method to Assess Non-mutagenic Carcinogens with iPS Cells
Self-renewal Potential of Cancer Stem Cells
Differentiation Potential of Cancer Stem Cells In Vitro
Tumor Angiogenesis by Cancer Stem Cells In Vivo
Invasion and Metastatic Potential of Cancer Stem Cells In Vitro
Metastatic Potential of Cancer Stem Cells In Vivo
Anchorage-Independent Cell Growth Assay for Cancer Stem Cells: Tumorigenic Assay in Vitro
Tumorigenic Potential of Cancer Stem Cells In Vivo
Development of Immunoliposomes Using Monoclonal Antibodies Targeting Cancer Stem Cells
In Vitro Evaluation of Anti-Cancer Stem Cell Drugs