Lundin Kersti, Ahlström Aisling (eds.) Manual of Embryo Culture in Human Assisted Reproduction

Cambridge University Press, 2021. — 141 p. — ISBN 978-1-108-81261-0.Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.Facilities for Embryo Culture
Incubators for Embryo Culture
Consumables for the IVF Laboratory: Production and Validation, Quality Control, and Bioassays
Embryo Metabolism and What Does the Embryo Need?
Culture Media and Embryo Culture
Optimal Handling Techniques for Culture of Human Embryos
From Identification to Witnessing: Traceability to Ensure the Safety of the Embryo during Culture
Timing of Embryo Culture
Time-Lapse Technology: Theoretical and Practical Aspects
Laboratory Monitoring for Embryo Culture
Embryo Culture and IVF Offspring Outcome
The Changing Culture of Embryo Culture: Advances in the IVF Culture System