Farrell R.E. RNA Methodologies: Laboratory Guide for Isolation and Characterization

5th Edition. — Academic Press, 2017 — 876 p. — ISBN: 978-0128046784.RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise.RNA and the Cellular Biochemistry Revisited
Creating a Ribonuclease-free Environment
RNA Isolation Strategies
The Truth about Tissues
Going Green: RNA and the Molecular Biology of Plants
Quality Control for RNA Preparations
cDNA: A Permanent Biochemical Record of the Cell
RT-PCR: A Science and an Art Form
Quantitative PCR Techniques
miRNA
RNA Interference - Take a RISC: Role the Dicer
Stringency: Conditions that Influence Nucleic Acid Structure
Electrophoresis of RNA
Photodocumentation and Image Analysis
Northern Analysis
Nucleic Acid Probe Technology
Isolation of Polyadenylated RNA
Quantification of Specific mRNAs by Nuclease Protection
Analysis of Nuclear RNA
Non-Array Methods for Global Analysis of Gene Expression
Genomes, Transcriptomes, Proteomes, and Bioinformatics
RNA Biomarker Development
High-Throughput Analysis of Gene Expression
Functional Genomics and Transcript Profiling
A Few RNA Success Stories