Mackay Ian M. (ed.) Real-Time PCR in Microbiology: From Diagnosis to Characterization

Caister Academic Press, 2007. — 465 p. — ISBN: 978-1-904455-18-9Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.Real-Time PCR: History and Fluorogenic Chemistries
Oligonucleotide Design for In-House Real-Time PCR Applications in Microbiology
QPCR: Target Preparation
Standards and Controls: Concepts for Preparation and Use in Real-Time PCR Applications
Quantification of Micro-Organisms: Not Human, Not Simple, Not Quick
Multiplex rtPCR in Microbiology
The Role of Real-time PCR in Routine Microbial Diagnostics
Challenges Facing Real-Time PCR Characteriztaion of Acute Respiratory Tract Infections
Rapid Detection of Bioterror Agents
Experts Roundtable: Real-Time PCR and Microbiology