Soares Michael J., Hunt Joan S. (eds.) Placenta and Trophoblast Methods and Protocols, Volume II

Humana Press, 2006. — 476 p. — (Methods in Molecular Medicine) — ISBN: 978-1-58829-608-5Placenta research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of the trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function. Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.Placenta and Trophoblast: Methods and Protocols: Overview II
In Vivo Analysis of Trophoblast Cell Invasion in the Human
In Vitro Analysis of Trophoblast Invasion
An In Vitro Model of Trophoblast Invasion of Spiral Arteries
In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells
Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface
Methods for Isolation of Cells from the Human Fetal-Maternal Interface
In Vitro Models for Studying Human Uterine and Placental Macrophages
Macrophage-Trophoblast Interactions
Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon
Analysis of the Soluble Isoforms of HLA-G
In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport
In Vitro Models for Studying Trophoblast Transcellular Transport
In Vitro Methods for Studying Human Placental Amino Acid Transport
Methods for Investigating Placental Fatty Acid Transport
Heterologous Expression Systems for Studying Placental Transporters
Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures
Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells
Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands
Bacterial Expression of Prolactin Family Proteins
Analysis of Placental Regulation of Hematopoiesis
Methods for Studying Interferon Tau Stimulated Genes
Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia
In Vivo Rat Model of Preeclampsia
A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice
Working with Oxygen and Oxidative Stress In Vitro
Hypobaric Hypoxia as a Tool to Study Pregnancy-Dependent Responses at the Maternal-Fetal Interface
Infection With Listeria monocytogenes as a Probe for Placental Immunological Function