Usha, Sonkar Gyanendra Kumar, Singh Sangeeta (eds.) Manual of Immunopathological Techniques

Jaypee Brothers Medical Publishers, 2014. — 191 p.Manual of Immunopathological Techniques is collection of chapters contributed by experienced immunologists/immunopathologists, all of whom have hands on expertise of techniques they described. The idea of this manual arose from observations that those working in clinical/research field needed at some time or another, to perform an immunological technique applied to their own specific field of research. The existing manuals in market are either too detailed or require background expertise that the user may not necessarily possess. This manual is intended for use in broad clinical/research field including immunology, immunopathology, pathology, biochemistry, animal and veterinary sciences, hematology and genetics of both medical and basic sciences. Manual of Immunopathological Techniques is collection of chapters contributed by experienced immunologists/immunopathologists, all of whom have hands on expertise of techniques they described. The idea of this manual arose from observations that those working in clinical/research field needed at some time or another, to perform an immunological technique applied to their own specific field of research. The existing manuals in market are either too detailed or require background expertise that the user may not necessarily possess. This manual is intended for use in broad clinical/research field including immunology, immunopathology, pathology, biochemistry, animal and veterinary sciences, hematology and genetics of both medical and basic sciences. In spite of much use of internet for getting one's answer related to different methodologies, this manual will definitely find a place in the laboratories as there is no such book available in the market to our best of knowledge. Each chapter provides readers with a brief introduction about the topic/technique, its principle followed by step-by-step description of methodology, interpretation of the results, colored photographs and lastly the preparations of reagents with points to remember.Tests for Cell Mediated Immunity (CMI)
Lymphocytes Typing by Flow Cytometry
Diagnosis of Kala-Azar by Polymerase Chain Reaction (PCR) Technique
Test for Myeloma Profile and Qualitative Assessment of Serum Proteins
Agarose Gel Electrophoresis for Qualitative Assessment of Serum Proteins
Cellulose Acetate Electrophoresis
Agar Gel Immunoelectrophoresis for Typing of Myeloma
Micro Agar Gel Double Diffusion Technique
Counter Current Immunoelectrophoresis
Single Radial Immunodiffusion and Immunoturbidometric Methods for the Estimation of Immunoglobulins
Estimation of Complement C3, C4 and Hemolytic Assay for Serum Complement
Demonstration of Immune Complexes and Complements by Indirect Immunoperoxidase Method
Test for Autoimmune Diseases
Test for Autoantibodies by Enzyme-Linked Immunosorbent Assay Technique
Acid Fuchsin Orange G in Kidney Biopsy for Demonstration of Immune Complexes
Detection of Tumor Markers in Paraffin-embedded Sections by Immunohistochemistry
Detection of Circulating Immune Complexes in the Serum
Demonstration of Immune Complexes, Antibodies and Complements in Renal Biopsy by Immunofluorescence
Human Leukocyte Antigen Typing and Crossmatch Test in Solid Organ Transplantation
Human Leukocyte Antigen B27 Typing by Polymerase Chain Reaction Method
Fluorescence In Situ Hybridization: Methods and Applications
Detection of Cytokines—Interleukin (IL)-6, Interleukin (IL)-8 and Tumor Necrosis Factor (TNF)-α
Western Blot Tests for Detection of Antibody to Extractable Nuclear Antigen
Detection of Antithyroglobulin Antibodies by Hemagglutination Method
Distillation and Demineralization of Water
Raising of Polyclonal Anti-human Anti-IgG in Rabbit