Allewell N.M., Narhi L.O., Rayment I. (Eds.) Molecular Biophysics for the Life Sciences

Springer, 2013. — 401 p.
This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines - DNA helicases, ATP synthases, and myosin - illustrate how the combination of theory and experiment leads to new insights and new questions.Introduction (by Norma M. Allewell, Linda O. Narhi, and Ivan Rayment).
Structural, Physical, and Chemical Principles (by Norma M. Allewell, Linda O. Narhi, and Ivan Rayment).
The Experimental Tools of Molecular Biophysics
Optical Spectroscopic Methods for the Analysis of Biological Macromolecules (by Linda O. Narhi, Cynthia H. Li, Ranjini Ramachander, Juraj Svitel, and Yijia Jiang).
Diffraction and Scattering by X-Rays and Neutrons (by Ivan Rayment).
Nuclear Magnetic Resonance Spectroscopy (by Thomas C. Pochapsky and Susan Sondej Pochapsky).
Electron Paramagnetic Resonance Spectroscopy (by John H. Golbeck and Art van der Est).
Mass Spectrometry (by Igor A. Kaltashov and Cedric E. Bobst).
Single-Molecule Methods (by Paul J. Bujalowski, Michael Sherman, and Andres F. Oberhauser).
Biological Macromolecules as Molecular Machines: Three Examples
Helicase Unwinding at the Replication Fork (by Divya Nandakumar and Smita S. Patel).
Rotary Motor ATPases (by Stephan Wilkens).
Biophysical Approaches to Understanding the Action of Myosin as a Molecular Machine (by Mihaly Kovacs and Andras Malnasi-Csizmadia).
Future Prospects
Future Prospects (by Norma M. Allewell, Igor A. Kaltashov, Linda O. Narhi, and Ivan Rayment).