Innocenti F., Van Schaik R.H.N. (Eds.). Pharmacogenomics: Methods and Protocols

2nd Edition. — Springer, 2013. — 375 p.Understanding an individual's genetic makeup is the key to creating personalized drugs with greater efficacy and safety, and pharmacogenomics aims to study the complex genetic basis of inter-patient variability in response to drug therapy. Based upon the success of its first edition, the second edition of Pharmacogenomics: Methods And Protocols aims to continue providing readers with high-quality content on the most innovative and commonly adopted technologies in the field of pharmacogenomics as presented by experts in the field. Broken into several sections, this detailed volume examines techniques for interrogating variation in human genes and genomes, functional assessment of genetic variation, both in vitro and in vivo, as well as tools for translation and implementation of pharmacogenetic markers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Introduction to Pharmacogenomics
Pharmacogenomics: Historical Perspective and Current Status
Techniques for Interrogating Variation in Human Genes and Genomes
Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping
Clinical SNP Detection by the SmartAmp Method
MALDI-TOF Mass Spectrometry
TaqMan Drug Metabolism Genotyping Assays for the Detection of Human Polymorphisms Involved in Drug Metabolism
Pyrosequencing of Clinically Relevant Polymorphisms
Pharmacogenetics Using Luminex xMAP Technology: Method for Developing a Custom Multiplex Single Nucleotide Polymorphism Mutation Assay
Use of Linkage Analysis, Genome-Wide Association Studies, and Next-Generation Sequencing in the Identification of Disease-Causing Mutations
The GoldenGate Genotyping Assay: Custom Design, Processing, and Data Analysis
Genome-Wide Gene Expression Profiling, Genotyping, and Copy Number Analyses of Acute Myeloid Leukemia Using Affymetrix GeneChips
Epigenetic Techniques in Pharmacogenetics
Plasmid Derived External Quality Controls for Genetic Testing
Functional Assessment of Genetic Variation: In Vitro and In Vivo Methods
Allelic Imbalance Assays to Quantify Allele-Specific Gene Expression and Transcription Factor Binding
SCAN: A Systems Biology Approach to Pharmacogenomic Discovery
Methods to Examine the Impact of Nonsynonymous SNPs on Protein Degradation and Function of Human ABC Transporter
In Vitro Identification of Cytochrome P450 Enzymes Responsible for Drug Metabolism
In Vitro and In Vivo Mouse Models for Pharmacogenetic Studies
The Hydrodynamic Tail Vein Assay as a Tool for the Study of Liver Promoters and Enhancers
Tools for Translation and Implementation of Pharmacogenetic Markers
A Guide to the Current Web-Based Resources in Pharmacogenomics
PharmGKB: The Pharmacogenomics Knowledge Base
Genetic Databases in Pharmacogenomics: The Frequency of Inherited Disorders Database (FINDbase)
Development of Predictive Models for Estimating Warfarin Maintenance Dose Based on Genetic and Clinical Factors
Evidence Based Drug Dosing and Pharmacotherapeutic Recommendations Per Genotype