Rosenthal S.J., Wright D.W. (Eds.) NanoBiotechnology Protocols

Humana Press, 2005. — 242 p.Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.Applications of Quantum Dots in Biology: An Overview
Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates
Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells
Quantum Dot-Encoded Beads
Use of Nanobarcodes Particles in Bioassays
Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging
Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies
Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters
Surface-Functionalized Nanoparticles for Controlled Drug Delivery
Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis
Structural DNA Nanotechnology: An Overview
Nanostructured DNA Templates
Probing DNA Structure With Nanoparticles
Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells
Real-Time Cell Dynamics With a Multianalyte Physiometer